Journal: Nanomedicine : nanotechnology, biology, and medicine

Article Title: Synergic antibacterial coatings combining titanium nanocolumns and tellurium nanorods.

doi: 10.1016/j.nano.2018.12.009

Figure Lengend Snippet: Fig. 6: E. coli colonies on the different samples after 16h. N=3. *p<0.005 versus control, **p<0.01 versus control.

Article Snippet: For the sake of comparison, the sputtered Ti thin films as well as commercial Ti disks from Goodfellow (thickness: 0.5 mm, code: 303-115-36) were also used: no significant differences between them were observed, so their results were averaged and jointly labeled D. Escherichia coli (strain K-12 HB101; Bio-Rad, Hercules, CA) and Staphylococcus aureus (subsp. aureus Rosenbach, ATCC® 12600TM; ATCC, Manassas, VA) bacteria were used.

Techniques: Control

Journal: The Journal of Biological Chemistry

Article Title: Tyrosine nitration on calmodulin enhances calcium-dependent association and activation of nitric-oxide synthase

doi: 10.1074/jbc.RA119.010999

Figure Lengend Snippet: Incorporation of nitroTyr into CaM expressed in E. coli. A, the human CaM construct with C-terminal His6 tag bearing either the native Tyr codon or an amber stop codon at amino acid position 99 or position 138 was co-transformed with a plasmid expressing an Methanocaldococcus jannaschii tyrosyl-tRNA synthetase/tRNACUA pair engineered to efficiently incorporate nitroTyr. B, WT– and nitroTyr–CaM was expressed in autoinduction medium with or without nitroTyr supplementation, purified using the C-terminal His6 tag, and analyzed by 15% SDS-PAGE gel. C, nitroTyr incorporation into CaM was determined here by Western blotting with a primary antibody against nitroTyr. D, electrospray ionization mass spectrometry confirms the quantitative incorporation of nitroTyr into CaM because all measured pure protein masses match their expected molecular masses.

Article Snippet: E. coli DH10B was transformed with pBad–CaM–(99 or 138 TAG) and pDule–nitroTyr–5B (Addgene plasmid no. 85498) ( 30 ).

Techniques: Construct, Transformation Assay, Plasmid Preparation, Expressing, Purification, SDS Page, Western Blot, Mass Spectrometry

Journal: Scientific Reports

Article Title: Fluorescence in situ hybridisation in Carnoy’s fixed tonsil tissue

doi: 10.1038/s41598-022-16309-w

Figure Lengend Snippet: Bacterial cell cultures used in this study to test and optimise FISH probes.

Article Snippet: Figure 3 Fluorescence in situ hybridisation staining of Streptococcus pyogenes ATCC 700,294, Pseudomonas aeruginosa ATCC BAA-47 and Escherichia coli K-12 DH5 alpha, ATCC PTA-4079 mixed cells from broth culture. ( A ) Nucleic material/DAPI in blue, ( B ) Eubacterial probe (EUB) in green, ( C ) Streptococcus spp . specific probe (STRC493) in red, ( D ) Pseudomonas spp . specific probe (PSE227) in yellow, ( E ) composite image of ( A – D ).

Techniques: