Journal: Journal of molecular biology

Article Title: A Generalizable Optogenetic Strategy to Regulate Receptor Tyrosine Kinases during Vertebrate Embryonic Development

doi: 10.1016/j.jmb.2020.03.032

Figure Lengend Snippet: Design and evaluation of the CMT-OptoTrk system. (a) Schematic of the CMT-OptoTrk constructs representing the two-step recruitment and dimerization model of activation explained in Figure 1 (a). Trk can be TrkA, TrkB, or TrkC. (b) Confocal microscopic images of live HEK293T cells grown on glass-bottomed petri dishes and transiently transfected with CMT-OptoTrkC. Ten pulses of 1-s blue laser light were delivered via the microscope objective, and the before and after images were recorded. Plasma membrane translocation of cytosolic TrkC after blue light is apparent in the red channel. The green channel visualizes the membrane-anchored CIBN-EGFP module, which marks the plasma membrane and seems unresponsive to blue light. (c) cFos luciferase assay for HEK293T cells in 24-well plates transiently co-transfected with plasmids encoding CMT-OptoTrks, cFOS promoter-controlled luciferase, and Renilla luciferase (control), and after illumination and processing as described in Figure 2(d). Values represent mean ± s.d. of three biological replicates. (d) CMT-OptoTrks induce robust PC12 cell differentiation with light. Representative images of CMT-OptoTrk- and CIBN-EGFP-CaaX(control)-transfected PC12 cells following incubation in the light or dark as explained in Figure 2(e). (e) Quantification of PC12 cell differentiation with CMT-OptoTrkA, -OptoTrkB, and -OptoTrkC as in Figure 2(e). The total number of counted cells is indicated on top of the bars.

Article Snippet: CMT-OptoTrkA, CMT-OptoTrkB, and CMT-OptoTrkC were made by inserting the rat TrkA intracellular domain (NCBI {"type":"entrez-protein","attrs":{"text":"NP_067600.1","term_id":"11024666","term_text":"NP_067600.1"}} NP_067600.1 ; residues 443–799), human TrkB intracellular domain (NCBI {"type":"entrez-protein","attrs":{"text":"NP_001018074.1","term_id":"65506745","term_text":"NP_001018074.1"}} NP_001018074.1 ; residues 455–822), and rat TrkC intracellular domain (NCBI {"type":"entrez-protein","attrs":{"text":"NP_001257585.1","term_id":"397174822","term_text":"NP_001257585.1"}} NP_001257585.1 ; residues 454–864), respectively, into the OptoRaf1 KpnI and Kpn2I sites by Infusion-cloning. pFOS WT-GL3 was a gift from Ron Prywes (Addgene plasmid no. 11983).

Techniques: Construct, Activation Assay, Transfection, Microscopy, Clinical Proteomics, Membrane, Translocation Assay, Luciferase, Control, Cell Differentiation, Incubation

Journal: Nanomedicine : nanotechnology, biology, and medicine

Article Title: Synergic antibacterial coatings combining titanium nanocolumns and tellurium nanorods.

doi: 10.1016/j.nano.2018.12.009

Figure Lengend Snippet: Fig. 6: E. coli colonies on the different samples after 16h. N=3. *p<0.005 versus control, **p<0.01 versus control.

Article Snippet: For the sake of comparison, the sputtered Ti thin films as well as commercial Ti disks from Goodfellow (thickness: 0.5 mm, code: 303-115-36) were also used: no significant differences between them were observed, so their results were averaged and jointly labeled D. Escherichia coli (strain K-12 HB101; Bio-Rad, Hercules, CA) and Staphylococcus aureus (subsp. aureus Rosenbach, ATCC® 12600TM; ATCC, Manassas, VA) bacteria were used.

Techniques: Control

Journal: Scientific Reports

Article Title: Fluorescence in situ hybridisation in Carnoy’s fixed tonsil tissue

doi: 10.1038/s41598-022-16309-w

Figure Lengend Snippet: Bacterial cell cultures used in this study to test and optimise FISH probes.

Article Snippet: Figure 3 Fluorescence in situ hybridisation staining of Streptococcus pyogenes ATCC 700,294, Pseudomonas aeruginosa ATCC BAA-47 and Escherichia coli K-12 DH5 alpha, ATCC PTA-4079 mixed cells from broth culture. ( A ) Nucleic material/DAPI in blue, ( B ) Eubacterial probe (EUB) in green, ( C ) Streptococcus spp . specific probe (STRC493) in red, ( D ) Pseudomonas spp . specific probe (PSE227) in yellow, ( E ) composite image of ( A – D ).

Techniques: